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Velocity Dna Polymerase Bio-21098
Minimum Order Quantity: 1 Piece
Brand | Duallife |
color | Black |
Material | Plastic |
Usage / Application | Hospital |
Packaging Type | Roll |
Country of Origin | Made in India |
- Accurate – possesses 3’ – 5’ proofreading exonuclease activity that delivers an error rate of 4.4 x 10-7 for excellent PCR fidelity
- Efficient – highly processive enzyme and advanced buffering system for increased PCR yield from even the most challenging templates
- Fast – extension rate of 1 5s/kb for ≤5 kb amplicons giving increased yield under fast thermal cycling conditions
- Robust – highly processive nature of the enzyme confers an increased tolerance to impurities and ability to amplify complex templates
- Flexible – ideal for high-fidelity amplification of targets up to 10 kb from human, animal and plant DNA
VELOCITY DNA Polymerase is recommended for high-fidelity PCR amplification. The enzyme possesses a 3’ – 5’ proofreading exonuclease activity that provides an exceptional error rate of 4.4 x 10-7 for highly accurate amplification from a very broad range of human, animal and plant targets. Furthermore, VELOCITY is a highly processive enzyme with extension rates as fast as 15 s/kb for targets up to 5 kb and 30 s/kb for targets up to 10 kb, thereby enabling a reduction in PCR turnaround times.
VELOCITY delivers exceptional fidelity with outstanding PCR yield even from low template concentrations. The increased processivity of VELOCITY, results in shorter extension times for fast PCR, increased product yield and the ability to amplify longer fragments. VELOCITY also offers robust and reliable product yields, even in assays where PCR conditions are challenging, including the presence of impurities or GC-rich targets. VELOCITY is a high-performance DNA polymerase, ideally suited for high-yield, fast PCR amplification of even long targets containing no mutations.
LED Digital 7 Hotplate, Glass Ceramic Hotplate, Heating Temperature Up To 550c With Pt1000
Heater Type | Ceramic |
Weight | 4.5kg |
Brand | DLAB |
Power | 1010W |
Voltage | 100-120/200-240V,50/60Hz |
• LED screen shows temperature
• Max. temperature up to 550°C
• Separate safety circuits with fixed safety temperature of 580°C
• External temperature control is possible by connecting the temperature sensor(PT 1000) with an accuracy at ±0.5°C
• Glass ceramic work plate provides excellent chemical-resistant performance and most efficient heat transfer
• The “HOT” warning will flash if the work plate temperature is above 50°C even when the hotplate is turned off
Specification
Specifications | HP550-S |
Work plate Dimension | 184x184mm(7 inch) |
Work plate material | Glass ceramic |
Power | 1010W |
Heating Power | 1000W |
Voltage | 100-120/200-240V,50/60Hz |
Heating position | 1 |
Heating temperature range | Room temp.-550°C,increment 5°C |
Control accuracy of work plate | ±10°C |
Safety temperature | 580°C |
Temperature display | LED |
Temperature display accuracy | ±1°C |
External temperature sensor | PT1000(±0.5°C) |
Heating warning | 50°C |
Protection class | IP21 |
Dimension [W x D x H] | 215x360x112mm |
Weight | 4.5kg |
Permissible ambient temperature and humidity | 5-40°C, 80%RH |
Tetro Reverse Transcriptase BIO-65050
Usage/Application | Hospital |
Brand | Duallife |
Cat No.- | Bio-65050 |
Material | Plastic |
Country Of Origin | India |
Tetro™ Reverse Transcriptase is a highly sensitive, high stability MMLV reverse transcriptase. Tetro Reverse Transcriptase is optimized for reverse transcription reactions using a wide range of total RNA amounts, such that long and low abundance cDNAs can be detected by amplification after cDNA synthesis.
Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, cDNA library construction, and the production of templates for RT-PCR analysis of gene expression.
Product Highlights- Highly sensitive - for high-quality, full length cDNA from as little as 10 pg of total RNA
- Ultra-stable - for long genes and rare transcripts
- High yield - produces high quality cDNA ideal for PCR
- Broad dynamic range - 10 pg to 5 μg of RNA
Tetro™ Reverse Transcriptase is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase, which exhibits high stability, with no loss of activity following 1 week at room temperature. Tetro Reverse Transcriptase is highly sensitive even when the amount of template is a limiting factor (fig. 1), with highly efficient and sensitive transcription, from as little as 10 pg, up to 5 μg of RNA (fig. 2).
Many RNA transcripts form stable secondary structures at lower temperatures, making them less suitable as templates for RT-PCR at those temperatures.
Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, with total RNA, mRNA and in vitro transcribed RNA and shows excellent performance with gene-specific primers, Oligo (dT) as well as random hexamers, making it perfect for cDNA library construction and the production of templates for RT-PCR analysis of gene expression.
RT-qPCR Extraction Control Orange MDX029 BIOLINE
Minimum Order Quantity: 1 Pack
Brand | Bioline |
Formerly RNA Extraction Control 560.
RNA Extraction Control not only enables users of a diagnostic qPCR assay to determine if there are inhibitors in the PCR assay, but also to validate the success of the extraction step, reducing the chance of obtaining a false negative result in the sample RNA.Product Highlights- Simple – streamlined protocol for straightforward validation of RNA extraction and determination of RT-qPCR assay inhibition
- Sensitive – control assay identifies even small effects on RNA extraction and inhibition of amplification
- Optimized - control RNA has a sequence with no known homology to any organism thereby avoiding detection of sample RNA
- Specific – probe-based assay designed specifically for the REC control sequence
- Flexible – ideal for use with a wide range of sample types, including inhibitor-rich samples like blood, urine and sputum samples
A common practice in qPCR is to add a known amount of spiked control RNA after RNA extraction, this monitors PCR inhibition but has no value as an extraction control. The ideal situation is to have the test sample and internal control undergo the same processing prior to qPCR. Meridian has developed a RT-qPCR Extraction Control, which more closely mimics the test sample, as compared to spike controls. Genetic material from the test sample and the RT-qPCR Extraction Control is simultaneously extracted by common extraction methods, with the extraction control being as sensitive to inhibition and extraction failure as the test sample.
Artificial RT-qPCR Extraction Control cells are of a known concentration, containing the Internal Control RNA sequence. This sequence contains no known homology to any organism and, importantly, has minimal interference with detection of sample RNA. The RT-qPCR Extraction Control cells are spiked into the lysis buffer with the target sample, prior to RNA extraction. Control Mix, which primers and probe, is then added to the reaction mix before amplification. Signal derived from the Internal Control RNA confirms the success of the extraction step. RT-qPCR Extraction Control also monitors co-purification of PCR inhibitors that may cause biased or false amplification patterns.
MDX029 500 Reactions
RT-qPCR Extraction Control Red MDX028
Minimum Order Quantity: 1 Pack
Brand | Bioline |
Formerly RNA Extraction Control 670.
RT-qPCR Extraction Control not only enables users of a diagnostic qPCR assay to determine if there are inhibitors in the PCR assay, but also to validate the success of the extraction step, reducing the chance of obtaining a false negative result in the sample RNA.Product Highlights- Simple – streamlined protocol for straightforward validation of RNA extraction and determination of RT-qPCR assay inhibition
- Sensitive – control assay identifies even small effects on RNA extraction and inhibition of amplification
- Optimized - control RNA has a sequence with no known homology to any organism thereby avoiding detection of sample RNA
- Specific – probe-based assay designed specifically for the REC control sequence
- Flexible – ideal for use with a wide range of sample types, including inhibitor-rich samples like blood, urine and sputum samples
A common practice in qPCR is to add a known amount of spiked control RNA after RNA extraction, this monitors PCR inhibition but has no value as an extraction control. The ideal situation is to have the test sample and internal control undergo the same processing prior to qPCR. Meridian has developed a RT-qPCR Extraction Control, which more closely mimics the test sample, as compared to spike controls. Genetic material from the test sample and the RT-qPCR Extraction Control is simultaneously extracted by common extraction methods, with the extraction control being as sensitive to inhibition and extraction failure as the test sample.
Artificial RT-qPCR Extraction Control cells are of a known concentration, containing the Internal Control RNA sequence. This sequence contains no known homology to any organism and, importantly, has minimal interference with detection of sample RNA. The RT-qPCR Extraction Control cells are spiked into the lysis buffer with the target sample, prior to RNA extraction. Control Mix, which includes primers and probe, is then added to the reaction mix before amplification. Signal derived from the Internal Control RNA confirms the success of the extraction step. RT-qPCR Extraction Control also monitors co-purification of PCR inhibitors that may cause biased or false amplification patterns.
qPCR Extraction Control Orange MDX027
Minimum Order Quantity: 1 Pack
Brand | Bioline |
Formerly DNA Extraction Control 560.
DNA Extraction Control not only enables users of a diagnostic qPCR assay to determine if there are inhibitors in the PCR assay, but also to validate the success of the extraction step, reducing the chance of obtaining a false negative result in the sample DNA.Product Highlights- Simple - streamlined protocol for straightforward validation of DNA extraction and determination of qPCR assay inhibition
- Sensitive - control assay identifies even small effects on DNA extraction and inhibition of amplification
- Optimized - control DNA has a sequence with no known homology to any organism thereby avoiding detection of sample DNA
- Specific - probe-based assay designed specifically for real-time PCR assays
- Flexible - ideal for use with a wide range of sample types, including inhibitor-rich samples like blood, urine and sputum samples
A common practice in qPCR is to add a known amount of spiked control DNA after DNA extraction, this monitors PCR inhibition but has no value as an extraction control. The ideal situation is to have the test sample and internal control undergo the same processing prior to qPCR. Meridian has developed the qPCR Extraction Control, which more closely mimics the test sample, as compared to spike controls. Genetic material from the test sample and the qPCR Extraction Control is simultaneously extracted by common extraction methods, with the extraction control being as sensitive to inhibition and extraction failure as the test sample.
The qPCR Extraction Control cells are of a known concentration, containing the Internal Control DNA sequence. This sequence contains no known homology to any organism and, importantly, has minimal interference with detection of sample DNA. The qPCR Extraction Control cells are spiked into the lysis buffer with the target sample, prior to DNA extraction. Control Mix, which includes primers and probe, is then added to the reaction mix before amplification. Signal derived from the Internal Control DNA confirms the success of the extraction step. qPCR Extraction Control also monitors co-purification of PCR inhibitors that may cause biased or false amplification patterns.
qPCR Extraction Control Red MDX026 BIOLINE
Minimum Order Quantity: 1 Pack
Brand | Bioline |
Formerly DNA Extraction Control 670.
qPCR Extraction Control not only enables users of a diagnostic qPCR assay to determine if there are inhibitors in the PCR assay, but also to validate the success of the extraction step, reducing the chance of obtaining a false negative result in the sample DNA.Product Highlights- Simple - streamlined protocol for straightforward validation of DNA extraction and determination of qPCR assay inhibition
- Sensitive - control assay identifies even small effects on DNA extraction and inhibition of amplification
- Optimized - control DNA has a sequence with no known homology to any organism thereby avoiding detection of sample DNA
- Specific - probe-based assay designed specifically for real-time PCR assays
- Flexible - ideal for use with a wide range of sample types, including inhibitor-rich samples like blood, urine and sputum samples
A common practice in qPCR is to add a known amount of spiked control DNA after DNA extraction, this monitors PCR inhibition but has no value as an extraction control. The ideal situation is to have the test sample and internal control undergo the same processing prior to qPCR. Meridian has developed the qPCR Extraction Control, which more closely mimics the test sample, as compared to spike controls. Genetic material from the test sample and the qPCR Extraction Control is simultaneously extracted by common extraction methods, with the extraction control being as sensitive to inhibition and extraction failure as the test sample.
The qPCR Extraction Control cells are of a known concentration, containing the Internal Control DNA sequence. This sequence contains no known homology to any organism and, importantly, has minimal interference with detection of sample DNA. The qPCR Extraction Control cells are spiked into the lysis buffer with the target sample, prior to DNA extraction. Control Mix, which includes primers and probe, is then added to the reaction mix before amplification. Signal derived from the Internal Control DNA confirms the success of the extraction step. qPCR Extraction Control also monitors co-purification of PCR inhibitors that may cause biased or false amplification patterns.
MDX0262000 Reactions
5X DNA Gel Loading Buffer
Product Brochure
Usage/Application | Hospital |
Brand | Duallife |
Material | PP |
Country Of Origin | India |
Pr17-8-Strip Tubes & Optically Clear Flat Caps For Real-time PCR 1x125no
Brand | HIMEDIA |
Model Name/Number | PR17 |
Sample Capacity/Format | 1*125 |
Volume Thermal Block Sample | 0.2 mL |
MangoMix BIO-25033
Brand | Bioline |
MangoMix™ is a convenient, ready-to-go, 2x Reaction Mix containing MangoTaq™ DNA Polymerase, MgCl2 and ultra-pure dNTPs manufactured by Meridian. The Mix is optimized and ready-to-use and requires only the addition of water, template and primers.
Product Highlights- Direct gel loading - no need for further post-PCR processing steps
- Easy visual recognition- reduces pipetting errors
- High performance - pre-optimized 2x solutions
- Ready to use format - reduces risk of contamination and decreases the reaction set-up time
- Reproducible results - consistent QC ensures reliability
MangoMix™ is optimized and ready-to-use, so the user need only add water, template and primers. MangoMix reduces the time required to set up reactions, thereby minimizing contamination risks and providing greater reproducibility through a reduction in the number of pipetting steps. MangoMix can be loaded directly onto an agarose gel for analysis, without the need for a separate gel-loading buffer.
The presence of dyes has no effect on routine enzymatic manipulations, although rare exceptions may exist. MangoMix has been optimized for a wide variety of templates. An additional 50 mM of MgCl2 solution is included should any fine adjustments be required.
RT PCR Machine
Brand | Duallife |
Communication Interface | USB |
Country of Origin | Made in India |
Usages | Hospital |
Material | Plastic |