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Molecular Biology Reagents

Offering you a complete choice of products which include diagnostic test kit, isolate ii rna plant kit bioline, isolate ii rna micro kit bioline, isolate ii rna mini kit bioline, isolate ii rna mini kit bio-52071 and pbs- phosphate-buffered saline.
Diagnostic Test Kit

Diagnostic Test Kit

Product Price: Rs 1 Lakh / PieceGet Best Price

Minimum Order Quantity: 1 Piece

Product Details:
BrandDuallife
ICMR ApprovedYes
Test Method(SARS-CoV-2) RT-PCR
Sample TypeBlood
Usage/ApplicationHospital
Shelf Life6 months
DiseaseDNA
Country of OriginMade in India
Cat No.BIO-39041
MaterialPlastic
Ready-to-use molecular grade dUTP Mix containing dATP, dCTP, dGTP and dUTP, designed to save hands-on time for researchers and minimize the possibility of contamination.Product Highlights
  • High quality - >99% purity determined by HPLC, ideal for use in PCR reactions
  • Long shelf-life - no need for aliquoting
  • Convenient formats - available in various concentrations, offering ease of use
  • Enzyme free - DNase, RNase and Nickase free
  • Flexible sizes - custom, bulk and OEM sizes available
Product Description

Highly quality dNTPs are& a vital requirement for successful PCR, as the presence of contaminating impurities will result in a decrease in amplification sensitivity and product yield. Meridian ultra-pure dNTPs undergo highly stringent purification steps to give a greater than 99% purity and are tested for the absence of DNase, RNase, Protease, Nickase activity. They are then quality controlled in a variety of applications, ensuring that they are highly suitable for the most sensitive of PCR techniques.

dUTP in the mix is used in place of dTTP and is used in PCR and qPCR protocols to prevent carryover from previous amplifications. Uracil DNA Glycosylase (UDG) is added to a PCR premix to catalyze the release of uracil from uracil-containing single-stranded or double-stranded DNA created in previous PCR reactions, thereby preventing false positives.

Meridian dUTP Mix are supplied as 10 mM of ultra-pure dATP, dCTP and dGTP and 20 mM dUTP solution as lithium salts. Lithium salts have greater resistance to repeated freezing and thawing cycles than sodium salts and remain sterile due to the bacteriostatic activity of lithium towards various microorganisms, giving greater reliability and an extended shelf life.

Cat. No.                Size
BIO-39041            25µmol (1 x 500µl)
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ISOLATE II RNA Plant Kit BIOLINE

ISOLATE II RNA Plant Kit BIOLINE

Product Price: Rs 1 Lakh / KitGet Best Price

Minimum Order Quantity: 1 Kit

Product Details:
BrandBioline
Highly efficient purification of total RNA by combining the stringency of either guanidinium thiocyanate or guanidinium hydrochloride with the speed and purity of silica-membrane column purification.Product Highlights

RNA was isolated from 20 mg freeze-dried budding leaves of A. thaliana using ISOLATE II RNA Plant Kit. The extracted RNA was diluted in a 2-fold serial dilution (lanes 1-7) and PCR was performed using MyTaq One-Step RT-PCR Kit. The results illustrate the quality of the RNA obtained, as it can be used for very sensitive cDNA synthesis and PCR without further purification.

Product Description

The ISOLATE II RNA Plant Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of plant materials, including leaves, bark, roots and fruits, without the need for hazardous reagents such as phenol.

By combining the stringency of guanidinium thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II RNA Plant Kit provides a fast method for the purification of high-quality total RNA from most plant cells, including plant tissues, leaves, bark, roots and fruits.

Some plant tissues such as maize endosperm and the mycelia of filamentous fungi do not lyse well in guanidinium thiocyanate and can solidify, resulting in poor yields and so guanidinium hydrochloride lysis buffer is also provided as an alternative. Residual amounts of contaminating DNA are selectively removed using a column, however for ultrasensitive applications, remaining DNA can be removed using RNase-free DNase I that is supplied with the kit. 

The ISOLATE II RNA Plant Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Plant Kit can be used to purify samples prior to RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Meridian PCR portfolio, including MyTaq DNA Polymerase.

Cat. No.                 Size
BIO-52076             10 Preps
BIO-52077             50 preps
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ISOLATE II RNA Micro Kit BIOLINE

ISOLATE II RNA Micro Kit BIOLINE

Product Price: Rs 1 Lakh / KitGet Best Price

Minimum Order Quantity: 1 Kit

Product Details:
BrandBioline
Highly efficient purification of total RNA from very small samples using an innovative silica-membrane column designed to elute in very small volumes, resulting in high RNA concentrations.Product Highlights
  • Fast – simple extraction of high purity total RNA from twelve samples, ideal for use in all applications, in as little as 40 minutes
  • High-performance – excellent recovery of high quality RNA from single cells, laser-captured or flow-sorted cells, cryosections and small tissue samples, including biopsies
  • Safe - no hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl or alcohol precipitation
  • Concentrated - small elution volumes ensure reaction volumes can be kept low in downstream applications, giving increased reaction efficiency
  • Convenient – includes all necessary components, including filters (shredders) and DNase I
Product Description

The ISOLATE II RNA Micro Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol.

By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II RNA Micro Kit provides a fast method for the purification of high-quality total RNA from single cells, pellets of cultured cells, cryosections, laser-captured or flow sorted cells and small amounts of tissues, for example biopsy materials.

For applications that are sensitive to very small amounts of DNA, residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment with the RNase-free DNase I that is supplied with the kit. 

The ISOLATE II RNA Micro Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Micro Kit can be used to purify samples prior to PCR and RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Meridian PCR portfolio, including MyTaq DNA Polymerase.

Cat. No.                   Size
BIO-52074               10 Preps
BIO-52075               50 Preps
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ISOLATE II RNA Mini Kit BIOLINE

ISOLATE II RNA Mini Kit BIOLINE

Product Price: Rs 1 Lakh / KitGet Best Price

Minimum Order Quantity: 1 Kit

Product Details:
BrandBioline
Highly efficient purification of total RNA by combining the stringency of guanidinium thiocyanate lysis with the speed and purity of silica-membrane column purification.Product Highlights
  • Fast – simple extraction of high purity total RNA from six samples, ideal for use in all applications, in as little as 30 minutes
  • High-performance – recovery of consistently high quality RNA from a wide variety of animal, bacterial and plant cells and tissues and cell-free samples
  • Efficient – optimized lysis conditions and column matrix for recovery of up to 70 mg of high purity RNA from each sample
  • Convenient – includes all necessary components, including filters (shredders) and DNase I
  • Safe - no hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl or alcohol precipitation
Product Description

The ISOLATE II RNA Mini Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol.

By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II RNA Mini Kit provides a fast method for the purification of high-quality total RNA from animal and plant cells and tissues as well as cultured cells, bacterial cells, yeast, biological fluids and cell-free samples.

Biological samples which are sometimes difficult to process i.e. mouse tissue (liver, brain), various tumor cell lines, Streptococcus and Actinobacillus pleuropneumoniae, will yield high-quality RNA with the ISOLATE II RNA Mini Kit.

The online product manual has protocols for purifying total RNA from cultured cells, tissues, yeast, bacteria, biological liquids, paraffin embedded tissue and RNAlater® treated samples. There is also a protocol for a convenient on-column DNase treatment, using RNase-free DNase I that is supplied with the kit, for applications that are sensitive to very small amounts of DNA.

The ISOLATE II RNA Mini Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Mini Kit can be used to purify samples for PCR and RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Meridian PCR portfolio, including MyTaq DNA Polymerase.

Cat. No.                          Size
BIO-52071                     10 Preps
BIO-52072                     50 Preps
BIO-52073                     250 Preps
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ISOLATE II RNA Mini Kit BIO-52071

ISOLATE II RNA Mini Kit BIO-52071

Product Price: Rs 1 Lakh / KitGet Best Price

Minimum Order Quantity: 1 Kit

Product Details:
BrandBioline
Highly efficient purification of total RNA by combining the stringency of guanidinium thiocyanate lysis with the speed and purity of silica-membrane column purification.Product Highlights
  • Fast – simple extraction of high purity total RNA from six samples, ideal for use in all applications, in as little as 30 minutes
  • High-performance – recovery of consistently high quality RNA from a wide variety of animal, bacterial and plant cells and tissues and cell-free samples
  • Efficient – optimized lysis conditions and column matrix for recovery of up to 70 mg of high purity RNA from each sample
  • Convenient – includes all necessary components, including filters (shredders) and DNase I
  • Safe - no hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl or alcohol precipitation
Product Description

The ISOLATE II RNA Mini Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol.

By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II RNA Mini Kit provides a fast method for the purification of high-quality total RNA from animal and plant cells and tissues as well as cultured cells, bacterial cells, yeast, biological fluids and cell-free samples.

Biological samples which are sometimes difficult to process i.e. mouse tissue (liver, brain), various tumor cell lines, Streptococcus and Actinobacillus pleuropneumoniae, will yield high-quality RNA with the ISOLATE II RNA Mini Kit.

The online product manual has protocols for purifying total RNA from cultured cells, tissues, yeast, bacteria, biological liquids, paraffin embedded tissue and RNAlater® treated samples. There is also a protocol for a convenient on-column DNase treatment, using RNase-free DNase I that is supplied with the kit, for applications that are sensitive to very small amounts of DNA.

The ISOLATE II RNA Mini Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Mini Kit can be used to purify samples for PCR and RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Meridian PCR portfolio, including MyTaq DNA Polymerase.

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PBS- Phosphate-Buffered Saline
  • PBS- Phosphate-Buffered Saline
  • PBS- Phosphate-Buffered Saline
  • PBS- Phosphate-Buffered Saline

PBS- Phosphate-Buffered Saline

Product Price: Rs 6,653 / BottleGet Best Price

Minimum Order Quantity: 1 Bottle

Product Details:
Purity>99%
GradeBio-Tech Grade
FormLiquid
BrandPMBIO
Usage/ApplicationCELL CULTURE, MOLECULAR BIOLOGY LABORATORY
Appearance (colour)COLORLESS

PBS-Phosphate-Buffered Saline
PBS solution is ready to use upon dilution (10X) to the desired concentration. It is a pH-adjusted blend solution of Sodium Chloride, Potassium Chloride, Monobasic Potassium Phosphate, Dibasic Sodium Phosphate. It is used for maintaining cells in a physiological pH range, osmoregulation. PBS also used for routine immunohistochemical test- ing, hematological and molecular biology. FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DI- AGNOSTIC PROCEDURES.

 



Additional Information:

  • Delivery Time: 7 days
  • Packaging Details: 500 ML
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